Find, Rapidly, Otus with Galaxy Solution

The user-friendly and Galaxy-supported pipeline FROGS analyses large sets of DNA amplicons sequences accurately and rapidly, essential for microbe community studies. It produces abundance table of OTUs and their taxonomic affiliation.

• FROGS was designed to support multiplexed and demultiplexed sequences.
• The preprocessing tool is dedicated to paired sequences merging, cleaning and dereplication.
• The clustering tool uses Swarm with a local clustering threshold, not a global clustering threshold like other software do.
• Chimera removal tool uses VSEARCH combined with an innovative chimera cross-validation.
• A filtering tool allows to remove noisy data.
• Affiliation tool returns taxonomic affiliation for each OTU using two methods with a unique multi-affiliation output.
• A lot of statistical results and numerous graphical illustrations are also produced.
• FROGS is designed for non-specialists thanks to its Galaxy interface, but is also available with command lines github.
• Its tools can be used independently, or as a workflow.
• It is portable on all Galaxy platforms with few informatics requirements and architecture dependencies.

FROGS was tested on many datasets

• the followed tabs show comparisons between FROGS, MOTHUR, UPARSE and QIIME, the popular pipelines.