The user-friendly and Galaxy-supported pipeline FROGS analyses large sets of DNA amplicons sequences accurately and rapidly, essential for microbe community studies.

FROGS was tested on many datasets

Standard Operation Procedure for amplicons
i.e. 16S, rpob, coi, 18S, ...
Standard Operation Procedure for data with unmergeable amplicons
i.e. ITS, rpb2, d1d2, ...
The publications :

Frédéric Escudié, Lucas Auer, Maria Bernard, Mahendra Mariadassou, Laurent Cauquil, Katia Vidal, Sarah Maman, Guillermina Hernandez-Raquet, Sylvie Combes, Géraldine Pascal; FROGS: Find, Rapidly, OTUs with Galaxy Solution, Bioinformatics, Volume 34, Issue 8, 15 April 2018, Pages 1287–1294

Maria Bernard, Olivier Rué, Mahendra Mariadassou and Géraldine Pascal; FROGS: a powerful tool to analyse the diversity of fungi with special management of internal transcribed spacers, Briefings in Bioinformatics 2021, 10.1093/bib/bbab318
To test FROGS
Install FROGS: conda or galaxy toolshed.

FROGS Formation documentations : formation_documentations.tar.gz FROGS Formation data : formation_data1.tar.gz and formation_data2.tar.gz

Play with FROGS on the Galaxy server of Toulouse
  • Request an account to genotoul bionfo platform via a form Genotoul Platform
  • Enter your credential (once for apache server connexion and a second times for galaxy platform connexion): Galaxy Server (on the top) /user /login
Redo FROGS'assessments with command lines for analyse 16S amplicons Get data built with sequences from utax and silva databanks:

Redo FROGS'assessments with command lines for analyse ITS amplicons Get data built with sequences from UNITES:
GNU GPLv3 (details)